Two types of longline sets, night and day, were evaluated for differences in catch composition in Seychelles waters. Night sets were set at dusk and retrieved at dawn and used shallow baskets targeting swordfish, while day sets were set at dawn and retrieved at dusk and used shallow and deep baskets to target tuna. The majority (75%) of species, both bycatch and market, were caught during day sets. The proportion of bycatch to target species decreased with depth. Lancetfish were the main bycatch species during day sets and sharks were the primary bycatch species during night sets.